New Step by Step Map For explain the principle of ultraviolet spectroscopy
Watch out although making ready the sample and pipetting it right into a cuvette or on to a microvolume System. The sample should be homogeneous.For example, benzene shows λmax at 256 nm, While aniline exhibits λmax at 280 nm. Consequently, the NH2 team acts as an auxochrome and triggers the shift of λmax to a larger worth.Sample and reference c